Department of Radiology

The Optical/Bioluminescence Sub-Core of the SAIF provides the capability to perform cellular and molecular non-invasive in-vivo bioluminescence, near-infrared fluorescence and Cerenkov imaging.

The instrumentation allows sensitive, non-invasive molecular imaging for a variety of applications including detection and quantification of various bioluminescent or fluorescent reporter-expressing cells or tissues (in culture or in small animals).

The facility currently houses a Perkin Elmer IVIS Lumina II, Perkin Elmer IVIS Lumina III, two Li-COR Pearl Trilogy Imagers and two Perkin Elmer IVIS Spectrums. The Facility offers assistance with experimental design, regulatory approval, troubleshooting, data management, analysis and display.

Instrumentation

  • Perkin Elmer IVIS Lumina II (John Morgan Building, Room B100)
  • Li-COR Pearl Trilogy (John Morgan Building, Room B100 and SCTR 1-133)
  • Perkin Elmer IVIS Spectrum (SCTR 1-133 and SCTR 6-125 behind SCXC barrier)
  • Perkin Elmer IVIS Lumina III (SCTR 6-123 behind SCXC barrier)

Services

  • Perkin Elmer IVIS Lumina II
    • In-vitro Imaging: cultured cell imaging, tissue/organ imaging
    • Bioluminescence imaging
    • Fluorescence imaging - GFP, DsRed, Cy5.5, ICG
  • Perkin Elmer IVIS Lumina III
    • Advanced in vitro and in vivo bioluminescence and fluorescence imaging system
    • Seven fluorescence excitation filters and 19 fluorescence emission filters
    • Wide-field lens adapter allowing imaging of up to five mice simultaneously
  • Perkin Elmer IVIS Spectrum
    • Advanced in vitro and in vivo bioluminescence and fluorescence imaging system
    • 10 fluorescence excitation filters and 20 fluorescence emission filters
    • Spectral unmixing and 3D imaging capability
  • LI-COR Pearl Trilogy
    • In vitro and in vivo fluorescence imaging
    • 700 nm, 800 nm channels - IRDye® 680, IRDye® 700DX, IRDye® 800RS, IRDye® 800CW, Alexa Fluor® 680, Alexa Fluor® 750, Cy5.5, Cy7
    • Simultaneous detection of 700 nm and 800 nm channels
  • Assistance provided per user request
    • Rodent anesthesia
    • Substrate injection
    • Animal shaving
    • Living Image®/Pearl Cam® acquisition
    • Data quantification and analysis
    • Transferring Living Image®/Pearl Cam® data to other file formats
    • General advice on experimental design or protocol plans
  • View the new SAIF Optical Core flyer

    Rates and Scheduling

    The core is set up to provide service at fee with no profit built in. The fees charged include costs for current personnel, operation, necessary equipment maintenance and upgrades, etc.

    • Rates: As of July 1, 2015, $120 per hour for both IVIS Spectrum scanners and the IVIS Lumina III scanner, $90 per hour for the IVIS Lumina II scanner and for both Li-COR Pearl Trilogy scanners. Data analysis and training is free of charge.

    Scheduling Calendar

    Contact Information

    Jim Delikatny, PhD
    Director, Optical Bioluminescence Sub-Core and Research Professor of Radiology
    Phone: 215-898-3105
    Email: delikatn@mail.med.upenn.edu

    Elizabeth Browning, PhD
    Technical Director, Optical Bioluminescence Sub-Core
    Phone: 215-746-1427
    Email: beliz@mail.med.upenn.edu

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