News Release

PHILADELPHIA – Lab-grown neural networks have the ability to replace lost axonal tracks in the brains of patients with severe head injuries, strokes or neurodegenerative diseases and can be safely delivered with minimal disruption to brain tissue, according to new research from Penn Medicine’s department of Neurosurgical Research. Their work is published in the Journal of Neural Engineering.

Complex brain function derives from the activity of populations of neurons – discrete processing centers – connected by long fibrous projections known as axons. When these connections are damaged, by injury or diseases such as a Parkinson’s or Alzheimer’s disease, they, unlike many other cells in the body, have very limited capacity to regenerate, thus permanently disrupting the body’s signal transmission and communication structure.

Senior author D. Kacy Cullen, PhD, an assistant professor of Neurosurgery and his team have been working to grow replacement connections, referred to as micro-tissue engineered neural networks (micro-TENNS), in the lab and test their ability to “wire-in” to replace broken axon pathways when implanted in the brain. Cullen’s team advanced the micro-TENNs to consist of discrete populations of mature cerebral cortical neurons spanned by long axonal projections within miniature hair-like structures. These micro-TENNs, initially developed in collaboration with Doug Smith, MD, Robert A. Groff Professor of Teaching and Research in Neurosurgery and director of the Center for Brain Injury and Repair, are the first transplantable neural networks that mimic the structure of brain pathways in a miniature form.

In a previous 2015 publication in Tissue Engineering, Cullen and colleagues showed that preformed micro- TENNS could be delivered into the brains of rats to form new brain architecture that simultaneously replaced neurons as well as long axonal projections. “The micro-TENNS formed synaptic connections to existing neural networks in the cerebral cortex and the thalamus – involved in sensory and motor processing – and maintained their axonal architecture for several weeks to structurally emulate long-distance axon connections,” Cullen reported. This work was the first to demonstrate that living micro-TENNS could successfully integrate into existing brain structures and reconstitute missing brain pathways, but, the team observed the need for improvement in how they were delivered to the brain, as this initial study required that the micro-TENNs be drawn into needles.

In response, the research team developed a new, less invasive delivery method by applying an ultra-thin coating to the micro-TENNs using a gel commonly found in food and biomedical products. This new biomaterial strategy allows the encapsulation of fully formed engineered neural networks for insertion into the brain without the use of a needle. “We searched for materials that could form a hard shell that would soften immediately following insertion to better match the mechanical properties of the native brain tissue,” Cullen said. This, the team hypothesized, would minimize the body’s reaction and improve the survival and integration of the neural networks. The additional coating was not detrimental to the number of surviving neurons, and the needleless method substantially reduces the implant footprint, suggesting that it would cause less damage and thus provide a more hospitable environment for implanted neurons to integrate with the brain’s existing nervous system. “Additional research is required to directly test micro-TENN neuron survival and integration for each of these insertion methods,” Cullen said. 

Cullen and team plan to perfect their processes and further integrate neuroscience and engineering to come up with unique ways to aid patients suffering from brain injury or common neurodegenerative diseases such as Parkinson’s and Alzheimer’s disease.

“We hope this regenerative medicine strategy will someday enable us to grow individualized neural networks that are tailored for each patient’s specific need,” he said, “ultimately to replace lost neural circuits and improve brain function.”   

Funding for this study was provided by the National Science Foundation (Graduate Research Fellowship DGE-1321851), NIH (T32-NS043126 and T32-GM007517), and the Department of Veterans Affairs (RR&D Merit Review #B1097-I). The University of Pennsylvania has intellectual property covering this technology, and Dr. Cullen is an inventor. 

Penn Medicine is one of the world’s leading academic medical centers, dedicated to the related missions of medical education, biomedical research, excellence in patient care, and community service. The organization consists of the University of Pennsylvania Health System and Penn’s Raymond and Ruth Perelman School of Medicine, founded in 1765 as the nation’s first medical school.

The Perelman School of Medicine is consistently among the nation's top recipients of funding from the National Institutes of Health, with $550 million awarded in the 2022 fiscal year. Home to a proud history of “firsts” in medicine, Penn Medicine teams have pioneered discoveries and innovations that have shaped modern medicine, including recent breakthroughs such as CAR T cell therapy for cancer and the mRNA technology used in COVID-19 vaccines.

The University of Pennsylvania Health System’s patient care facilities stretch from the Susquehanna River in Pennsylvania to the New Jersey shore. These include the Hospital of the University of Pennsylvania, Penn Presbyterian Medical Center, Chester County Hospital, Lancaster General Health, Penn Medicine Princeton Health, and Pennsylvania Hospital—the nation’s first hospital, founded in 1751. Additional facilities and enterprises include Good Shepherd Penn Partners, Penn Medicine at Home, Lancaster Behavioral Health Hospital, and Princeton House Behavioral Health, among others.

Penn Medicine is an $11.1 billion enterprise powered by more than 49,000 talented faculty and staff.

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