Description of Research Expertise

Research in my laboratory is focused on T cell biology and applications of genetically-engineered T cells to the treatment of disease.

Active research projects in my laboratory include:

1) Developing chimeric antigen receptors (CARs) and other engineering approaches for enhancing the function and improved safety of adoptive T cell therapy.

2) Developing and applying synthetic immunoreceptors to the treatment of immune-mediated disease.

3) Exploring the role of co-stimulatory signals in directing T cell metabolism and the way this metabolism affects T cell function within tumors.

Selected Publications

Dhodapkar KM, Cohen AD, Kaushal A, Garfall AL, Manalo RJ, Carr AR, McCachren SS, Stadtmauer EA, Lacey SF, Melenhorst JJ, June CH, Milone MC, Dhodapkar MV.: Changes in Bone Marrow Tumor and Immune Cells Correlate with Durability of Remissions Following BCMA CAR T Therapy in Myeloma. Blood Cancer Discov. : 2022.

Michael C. Milone and Leslie Shaw: Therapeutic Drug Monitoring Teitz Textbook of Clinical Chemistry and Molecular Diagnostics, 7th ed. : 2022.

Gill, E. L., Mack, E. A., Osterhoudt, K. C., Shaw, L. M., Milone, M. C.: An Epidemic within a Pandemic: An 8-Year-Old Boy with Xylazine-Complicating Fentanyl Poisoning, and Drug Detection Challenges J Appl Lab Med : 2022.

Ghassemi, Saba, Durgin, Joseph S., Nunez-Cruz, Selene, Patel, Jai, Leferovich, John, Pinzone, Marilia, Shen, Feng, Cummins, Katherine D., Plesa, Gabriela, Cantu, Vito Adrian, Reddy, Shantan, Bushman, Frederic D., Gill, Saar I., O’Doherty, Una, O’Connor, Roddy S., Milone, Michael C.: Rapid manufacturing of non-activated potent CAR T cells Nature Biomedical Engineering : 2022.

Zhang, Q., Yang, J., Manoharan, Enea, Yu, A. B., Milone, M. C.: CRISPR/Cas9-mediated Gene Knockout Followed by Negative Selection Leads to a Complete TCR Depletion in ortho CAR19 T Cells Bio Protoc 12 (15): 2022.

Zhang, H., Yu, P., Tomar, V. S., Chen, X., Atherton, M. J., Lu, Z., Zhang, H. G., Li, S., Ortiz, A., Gui, J., Leu, N. A., Yan, F., Blanco, A., Meyer-Ficca, M. L., Meyer, R. G., Beiting, D. P., Li, J., Nunez-Cruz, S., O'Connor, R. S., Johnson, L. R., Minn, A. J., George, S. S., Koumenis, C., Diehl, J. A., Milone, M. C., Zheng, H., Fuchs, S. Y.: Targeting PARP11 to avert immunosuppression and improve CAR T therapy in solid tumors Nat Cancer 3 (7): 808-820,2022.

Song, E. Z., Wang, X., Philipson, B. I., Zhang, Q., Thokala, R., Zhang, L., Assenmacher, C. A., Binder, Z. A., Ming, G. L., O'Rourke, D. M., Song, H., Milone, M. C.: The IAP antagonist birinapant enhances chimeric antigen receptor T cell therapy for glioblastoma by overcoming antigen heterogeneity Mol Ther Oncolytics 27 : 288-304,2022.

Garfall, A. L., Cohen, A. D., Susanibar-Adaniya, S. P., Hwang, W. T., Vogl, D. T., Waxman, A. J., Lacey, S. F., Gonzalez, V. E., Fraietta, J. A., Gupta, M., Kulikovskaya, I., Tian, L., Chen, F., Koterba, N., Bartoszek, R. L., Patchin, M., Xu, R., Plesa, G., Siegel, D. L., Brennan, A., Nelson, A. M., Ferthio, R., Cosey, A., Shea, K. M., Leskowitz, R., Four, M., Wilson, W. V., Miao, F., Lancaster, E., Carreno, B. M., Linette, G. P., Hexner, E. O., Young, R. M., Bu, D., Mansfield, K. G., Brogdon, J. L., June, C. H., Milone, M. C., Stadtmauer, E. A.: Anti-BCMA/CD19 CAR T cells with early immunomodulatory maintenance for multiple myeloma responding to initial or later-line therapy Blood Cancer Discov : 2022.

Ellis, G. I., Deng, M. Z., Winn, D. W., Coker, K. E., Shukla, D., Bhoj, V., Milone, M. C., Duran-Struuck, R., Riley, J. L.: Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation STAR Protoc 3 (4): 101784,2022.

Ellis, G. I., Coker, K. E., Winn, D. W., Deng, M. Z., Shukla, D., Bhoj, V., Milone, M. C., Wang, W., Liu, C., Naji, A., Duran-Struuck, R., Riley, J. L.: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque Cell Rep Med 3 (5): 100614,2022.

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Academic Contact Information

3400 Spruce Street 7103 Founders Pavilion
Philadelphia, PA 19104
Phone: 215-662-6575