Description of Research Expertise:
Research Interest Summary:
The main interest of this group is the non invasive detection of metabolic changes that occur during tumor development and in response to anticancer drugs in models of human breast cancer. We employ a number of imaging modalities that include near infrared fluorescence optical imaging, MRI and MRS, PET and Cerenkov imaging. We have an active program in the design, synthesis and characterization of NIR optical contrast agents for the detection of phospholipase and choline kinase expression and activity in tumors and for the detection of pH and redox changes in the tumor microenvironment. Changes in lipid metabolite levels are monitored using MRS to determine potential biochemical markers for the detection of tumors and their early response to therapy and correlated with near-infrared optical imaging using enzyme-activated fluorescent contrast agents to detect enzyme activity in situ. We are pioneering the development of Cerenkov imaging by creating novel radiolabeled probes suitable for determining both location and function using dual PET and optical imaging.
Alejandro D. Arroyo, Andrea E. Guzmán, Alexander V. Kachur, Sarah J.Saylor, Anatoliy V.Popov, E. James Delikatny: Development of fluorinated naphthofluoresceins for Cerenkov imaging Journal of Fluorine Chemistry 225 : 27-34,2019.
Predina JD, Newton AD, Corbett C, Shin M, Sulfyok LF, Okusanya OT, Delikatny EJ, Nie S, Gaughan C, Jarrar D, Pechet T, Kucharczuk JC, and Singhal S: Near-infrared intraoperative imaging for minimally invasive pulmonary metastasectomy for sarcomas.
J Thorac Cardiovasc Surg. 157 (5): 2061-2069,2019.
Chiorazzo MG, Tunset HM, Popov AV, Johansen B, Moestue S, Delikatny EJ.: Detection and Differentiation of Breast Cancer Sub-Types using a cPLA2α Activatable Fluorophore Scientific Reports 16 (9): 6122,2019.
Newton AD, Predina JD, Corbett CJ, Frenzel-Sulyok LG, Xia L, Petersson EJ, Tsourkas A, Nie S, Delikatny EJ, Singhal S: Optimization of Second Window Indocyanine Green for Intraoperative Near-Infrared Imaging of Thoracic Malignancy J Am Coll Surg. 228 (2): 188-197,2019.
Liebov, B.K., Arroyo, A.D., Rubtsova, N.I., Osharovich, S.A., Delikatny, E.J. and Popov, A.V.: Nonprotecting group synthesis of a phospholipase C activatable probe with an azo-free quencher.
ACS Omega 30 (3): 6867-6873,2018.
Buljan, V.A., Graeber, M.B., Holsinger, R.M.D., Brown, D., Hambly, B.D., Delikatny, E.J., Vuletic, V.R., Krebs, X.N., Tomas, I.B., Bohorquez-Florez, J.J., Liu, G.J. and Banati, R.B.: Calcium-axonemal microtubuli interactions underlie mechanism(s) of primary cilia morphological changes.
J. Biol. Phys. 44 (1): 53-80,2018.
Kall, S.L., Delikatny, E.J. and Lavie. A.: Identification of a unique inhibitor-binding Site on Choline Kinase α.
Biochemistry 57 (8): 1316-1325,2018.
Predina, J.D., Newton, A.D., Keating, J., Barbosa, E.M. Jr, Okusanya, O., Xia, L., Dunbar, A., Connolly, C., Baldassari, M.P., Mizelle, J., Delikatny, E.J., Kucharczuk, J.C., Deshpande, C., Kularatne, S.A., Low, P., Drebin, J. and Singhal S.: Intraoperative molecular imaging combined with Positron Emission Tomography improves surgical management of peripheral malignant pulmonary nodules.
Annals of Surgery 266 (3): 479-488,2017.
Kachur, A.V., Arroyo, A.D., Bryan, N.W., Saylor, S.J., Popov, A.V. and Delikatny, E.J.: Synthesis of pH indicators for Cerenkov imaging by electrophilic substitution of bromine by fluorine in an aromatic system.
Journal of Fluorine Chemistry 200 : 146-152,2017.
Arlauckas, S.P., Kumar, M., Popov, A.V., Poptani, H. and Delikatny, E.J.: Near infrared fluorescent imaging of choline kinase alpha expression and inhibition in breast tumors Oncotarget 8 (10): 16518-30,2017.
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