Description of Research Expertise:
Dr. Weissman's laboratory focuses on the study of RNA and innate immune system biology and the application of these findings to vaccine research and gene therapy. There are three main projects in his laboratory. The first project began through the use of mRNA encoding antigen as a delivery system to load dendritic cells to promote broad immune responses as part of a vaccine. This project has expanded to include basic studies of RNA immunogenicity and translation and the development of applications for gene therapy. The second project develops new HIV envelope immunogens that can induce broad responses and cross-reactive neutralizing antibodies. The third project continues previous studies that identified a protein found on DC, macrophages, and epithelial cells that binds HIV envelope with high affinity. The main focus of this project is testing whether this and related molecules function in vivo to promote HIV genital tract infection.
Parhiz H, Shuvaev VV, Pardi N, Khoshnejad M, Kiseleva RY, Brenner JS, Uhler T, Tuyishime S, Mui BL, Tam YK, Madden TD, Hope MJ, Weissman D, Muzykantov VR: PECAM-1 directed re-targeting of exogenous mRNA providing two orders of magnitude enhancement of vascular delivery and expression in lungs independent of apolipoprotein E-mediated uptake J Control Release : pii: S0168-3659(18)30586-8,2018.
Foster JB, Choudhari N, Perazzelli J, Storm J, Hofmann TJ, Jain P, Storm PB, Pardi N, Weissman D, Waanders AJ, Grupp SA, Karikó K, Resnick AC, Barrett DM: Purification of mRNA Encoding Chimeric Antigen Receptor Is Critical for Generation of a Robust T-Cell Response Hum Gene Ther : 145,2018.
Pardi N, Parkhouse K, Kirkpatrick E, McMahon M, Zost SJ, Mui BL, Tam YK, Karikó K, Barbosa CJ, Madden TD, Hope MJ, Krammer F, Hensley SE, Weissman D: Nucleoside-modified mRNA immunization elicits influenza virus hemagglutinin stalk-specific antibodies Nat Commun 9 (1): 3361,2018.
Pardi N, Hogan MJ, Naradikian MS, Parkhouse K, Cain DW, Jones L, Moody MA, Verkerke HP, Myles A, Montefiori DC, Lobby JL, Saunders KO, Liao H-X, Korber BT, Sutherland KK, Scearce RM, Hraber PT, Muramatsu H, Ni H, Balikov DA, Lee C, Mui BL, Tam YK, Krammer F, Karikó K, Eisenlohr LC, Madden TD, Hope JM, Lewis MG, Lee KK, Hensley SE, Cancro MP, Haynes BF, Weissman D: Nucleoside-modified mRNA immunization elicits potent T follicular helper cell responses J Exp Med 215 (6): 1571-1588,2018.
Pardi Nt, Hogan MJ, Naradikian MS, Parkhouse K, Cain DW, Jones L, Moody MA, Verkerke HP, Myles A, Willis E, LaBranche CC, Montefiori DC, Lobby JL, Saunders KO, Liao H-X, Korber BT, Sutherland LL, Scearce RM, Hraber PT, Tombácz I, Muramatsu H, Ni H, Balikov DA, Li C, Mui BL, Tam YK, Krammer F, Karikó K, Polacino P, Eisenlohr LC, Madden TD, Hope MJ, Lewis MG, Lee KK, Hu S-L, Hensley SE, Cancro MP, Haynes BF, Weissman D: Nucleoside-modified mRNA vaccines induce potent T follicular helper and germinal center B cell responses J Exp Med 215 (6): 1571-1588,2018.
Ravimohan S, Nfanyana K, Tamuhla N, Tiemessen CT, Weissman D, Bisson GP: Common Variation in Is Associated With Early Death and Elevated Inflammasome Biomarkers Among Advanced HIV/TB Co-infected Patients in Botswana Open Forum Infect Dis 5 (5): ofy075,2018.
Pardi N, Hogan MJ, Porter FW, Weissman D: mRNA vaccines - a new era in vaccinology Nat Rev Drug Discov 17 (4): 261-279,2018.
Ravimohan S, Kornfeld H, Weissman D, Bisson GP: Tuberculosis and lung damage: from epidemiology to pathophysiology Eur Respir Rev 27 (147): pii: 170077,2018.
Hogan MJ, Conde-Motter A, Jordan APO, Yang L, Cleveland B, Guo W, Romano J, Ni H, Pardi N, LaBranche CC, Montefiori DC, Hu S-L, Hoxie JA, Weissman D: Increased surface expression of HIV-1 envelope is associated with improved antibody response in vaccinia prime/protein boost immunization Virology 514 : 106-117,2018.
Vinnard C, Ravimohan S, Tamuhla N, Pasipanodya J, Srivastava S, Modongo C, Zetola NM, Weissman D, Gumbo T, Bisson GP: Pyrazinamide clearance is impaired among HIV/tuberculosis patients with high levels of systemic immune activation PLoS One 12 (11): e0187624,2017.
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