PHILADELPHIA – A high-fat-diet-induced immune reaction causes inflammation leading to intestinal cancer in a mouse model – even among animals that are not obese -- according to a new study from the Perelman School of Medicine at the University of Pennsylvania, Case Western Reserve University, the Pacific Northwest Research Institute (PNRI), and others. Epidemiological and clinical evidence have linked obesity with inflammation and increased risk of cancer. Up to now, however, the molecular mechanisms linking these three conditions have been elusive. The interdisciplinary team published their findings in Molecular Cancer Research.
“We found that specific types of high-fat diets – based on corn or coconut oils like those found in certain salad dressings and ice cream -- are associated with increased tumor formation in a mouse model of intestinal cancer,” said co-senior author John Lambris, PhD, the Dr. Ralph and Sallie Weaver Professor of Research Medicine at Penn. “This model is particularly interesting because it resembles human familial adenomatous polyposis, a condition that carries an 80 percent risk of developing colorectal cancer in individuals with mutations in a tumor suppressor gene called Apc.”
While increased tumor formation was associated with mice fed corn or coconut fats, mice fed diets with olive oil as a source of fat did not develop intestinal polyps, despite being obese. “This observation led us to our first important conclusion that diet, but not necessarily obesity, can promote intestinal cancer,” said co-author Edimara Reis, PhD, a research associate in the Department of Pathology and Laboratory Medicine. In fact in late September, the FDA decided to give the food industry three years to phase out partially hydrogenated oils, the main source of trans fat in some foods based on corn oil and other types of fat.
Using the mouse model, which was developed by co-senior author Joseph Nadeau from PNRI and first author Stephanie Doerner from Case Western, the team concluded that a high-fat diet (rather than metabolic status) and the chemical composition of the diet are the most important factors for determining cancer risk. "Our results clearly show that eating a high-fat diet is sufficient to increase cancer risk, regardless of obesity,” said co-senior author Nadeau.
The team also noted that tumor formation was caused by inflammation induced by the corn- and coconut-based diets. Notably, inflammation and intestinal tumors in the mice were triggered very soon – three days -- after being fed these high-fat diets.
Specifically, the corn- and coconut-based HFDs, not the olive oil-based diet, activated the complement system, an arm of the innate immune system. Complement is an evolutionarily conserved network of proteins that regulate immune responses that destroy foreign invaders. The system is a rapid defense mechanism in most species, from primitive sponges to humans. Complement also has inflammatory functions, and the Lambris lab has been investigating C5aR (the C5a receptor) for more than two decades. In the current study, C5a, a related pro-inflammatory molecule, increased in the intestine and systemically in mice fed corn- and coconut-based HFDs.
“When mice were given a drug to reduce the activity of C5aR, it prevented the development of intestinal tumorigenesis in mice fed corn- and coconut HFDs, indicating that anti-complement therapy could act as an adjuvant with cancer treatments,” Lambris said. The team also surmises that a diet change and complement inhibitors could potentially be beneficial to people with the apc mutation to prevent carcinogenesis.
The team is now working on understanding the exact biochemistry of how certain HFDs activate complement with the production of the C5a molecule.
This work was funded by the National Institutes of Health (U54 CA116867, AI030040, AI068730, P40 RR012305).
Penn Medicine is one of the world’s leading academic medical centers, dedicated to the related missions of medical education, biomedical research, and excellence in patient care. Penn Medicine consists of the Raymond and Ruth Perelman School of Medicine at the University of Pennsylvania (founded in 1765 as the nation's first medical school) and the University of Pennsylvania Health System, which together form a $7.8 billion enterprise.
The Perelman School of Medicine has been ranked among the top medical schools in the United States for more than 20 years, according to U.S. News & World Report’s survey of research-oriented medical schools. The School is consistently among the nation’s top recipients of funding from the National Institutes of Health, with $405 million awarded in the 2017 fiscal year.
The University of Pennsylvania Health System’s patient care facilities include: The Hospital of the University of Pennsylvania and Penn Presbyterian Medical Center — which are recognized as one of the nation’s top “Honor Roll” hospitals by U.S. News & World Report — Chester County Hospital; Lancaster General Health; Penn Medicine Princeton Health; Penn Wissahickon Hospice; and Pennsylvania Hospital – the nation’s first hospital, founded in 1751. Additional affiliated inpatient care facilities and services throughout the Philadelphia region include Good Shepherd Penn Partners, a partnership between Good Shepherd Rehabilitation Network and Penn Medicine, and Princeton House Behavioral Health, a leading provider of highly skilled and compassionate behavioral healthcare.
Penn Medicine is committed to improving lives and health through a variety of community-based programs and activities. In fiscal year 2017, Penn Medicine provided $500 million to benefit our community.