What is a lymph node biopsy?

A lymph node biopsy is a procedure to remove some or all of a lymph node for testing. You have hundreds of these tiny organs throughout your body. They make infection-fighting white blood cells and filter lymph fluid that flows through your lymphatic system. Lymph nodes trap germs, like bacteria and viruses, that cause illnesses. Cancer cells that have spread from a primary tumor (metastasized) can also be found in lymph nodes.

At Penn Medicine, experts from a wide variety of specialties use lymph node biopsies to test for diseases or to diagnose or stage cancer. Our surgeons and radiologists perform these procedures regularly, often using less invasive techniques. After the biopsy, you’ll receive the results promptly so that you can make informed decisions with your doctor on the next steps in your care.

Why you might need a lymph node biopsy

Your doctor may recommend taking a tissue sample from your lymph node if the node is swollen and the swelling isn’t going away, or if an imaging test like a mammogram or ultrasound found an abnormality in the node. If you’ve been diagnosed with cancer, testing tissue samples from a nearby lymph node can determine if the cancer has spread.

A lymph node biopsy can help diagnose:

  • A serious infection
  • Conditions that cause an immune system overreaction, like sarcoidosis
  • Cancer of the lymphatic system, known as lymphoma
  • Cancer that has spread beyond a primary tumor

What happens during a lymph node biopsy?

Common areas for lymph node biopsies include your armpit, neck, groin, or belly. Your doctor will give you instructions before your procedure on how to prepare for the appointment. Depending on the type of biopsy being done, you may be given sedation to help you relax, a local anesthetic to numb the area, or general anesthesia to put you to sleep. Computed tomography (CT) scans, magnetic resonance imaging (MRI), or ultrasounds may be used before or during the biopsy to help guide your doctor to the lymph node.

Lymph node biopsy recovery

If you had a needle biopsy, you’ll go home soon after the procedure, but you should rest for the remainder of the day. If your doctor made an incision during your biopsy, you may need to stay in the hospital for a few hours before going home. Your health care team will let you know how to care for the wound and when the stitches will be removed. If you had a laparoscopic biopsy, there may be a drainage tube at the surgical site and you’ll receive instructions on how to care for it until it’s removed.

Risks of lymph node biopsies

It’s common to experience minor discomfort, swelling, and bruising after a lymph node biopsy. You may also have some bleeding. Contact your doctor if bleeding or pain gets worse or if the area around the biopsy becomes red. There’s a low risk of developing an infection, which can be treated with antibiotics. Any numbness should go away within a couple of months.

What happens if a lymph node biopsy is positive?

The cells collected during your biopsy are sent to a lab for a pathologist to examine under a microscope, and a report is sent to your health care team. A positive result means you have the disease for which you were tested. A lymph node malignancy means cancer cells were found in the lymph node.

Penn Medicine’s experts are here to help you understand your diagnosis and treat the condition with leading therapies. Our providers have expertise across all specialties, whether your biopsy was to test for lymphoma, cancer that’s spreading, an infection, or another disease. Lymph node biopsies are safe and effective tools in your care journey.

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