Description of Research Expertise:
Norovirus (NV) are major global pathogens associated with significant morbidity and mortality, yet there is no effective NV vaccine and we know little about NV immunity. Candidate vaccines have been ineffective despite eliciting strong antibody responses. Furthermore, NVs can cause persistent infections, challenging the long-held view that they are transient pathogens. Until recently there was little known about cellular immunity to NVs. Our research has focused on defining and tracking epitope-specific CD8 T cell responses during infection with acute and chronic strains of murine NV (MNV) to gain insight into mechanisms of viral clearance vs. persistence.
We used the MNV system to screen overlapping peptide libraries and define MHC class I and class II epitopes. We then tracked MNV specific CD8 T cells using MHC class I-peptide tetramers and uncovered key differences between T cell responses in acute vs. chronic MNV infection.
Vesselin T. Tomov, Olesya Palko, Chi Wai Lau, Yuhang Sun, Ralitza Tacheva, Bertram Bengsch, Sasikanth Manne, Gabriela L. Cosma, Laurence Eisenlohr, Timothy J. Nice, Herbert W. Virgin, and E. John Wherry: Enteric MNV persistence is associated with a unique CD8 T cell differentiation state and rapid immune evasion Keystone Symposium : 2017.
Nice TJ, Osborne LC, Tomov VT, Artis D, Wherry EJ, Virgin HW: Type I Interferon Receptor Deficiency in Dendritic Cells Facilitates Systemic Murine Norovirus Persistence Despite Enhanced Adaptive Immunity PLos Pathogen 12 (6): e1005684,2016.
Vesselin Tomov, Olesya Palko, and E. John Wherry: Human Norovirus-specific T Cell Responses Center for Molecular Studies in Digestive and Liver Diseases at the University of Pennsylvania 2015 Symposium : 2015.
Osborne Lisa C, Monticelli Laurel A, Nice Timothy J, Sutherland Tara E, Siracusa Mark C, Hepworth Matthew R, Tomov Vesselin T, Kobuley Dmytro, Tran Sara V, Bittinger Kyle, Bailey Aubrey G, Laughlin Alice L, Boucher Jean-Luc, Wherry E John, Bushman Frederic D, Allen Judith E, Virgin Herbert W, Artis David: Virus-helminth coinfection reveals a microbiota-independent mechanism of immunomodulation. Science (New York, NY) 345 (6196): 578-82,2014.
Bewtra Meenakshi, Brensinger Colleen M, Tomov Vesselin T, Hoang Tram B, Sokach Carly E, Siegel Corey A, Lewis James D: An optimized patient-reported ulcerative colitis disease activity measure derived from the Mayo score and the simple clinical colitis activity index. Inflammatory Bowel Diseases 20 (6): 1070-8,2014.
Tomov Vesselin T, Osborne Lisa C, Dolfi Douglas V, Sonnenberg Gregory F, Monticelli Laurel A, Mansfield Kathleen, Virgin Herbert W, Artis David, Wherry E John: Persistent enteric murine norovirus infection is associated with functionally suboptimal virus-specific CD8 T cell responses. Journal of virology 87 (12): 7015-31,2013.
Vesselin T. Tomov, Lisa C. Osborne, Douglas V. Dolfi, Gregory F. Sonnenberg, Laurel A. Monticelli, Kathleen Mansfield, Herbert W. Virgin, David Artis, E. John Wherry: Persistent enteric murine norovirus infection is associated with functionally suboptimal virus-specific CD8 T cell responses American Association of Immunologists 2013 Annual Conference : 2013.
Vesselin T. Tomov, Olesya Palko, Lisa Osborne, Yuhang Sun, David Artis, and E. John Wherry: Epitope-specific CD8 T cell subsets mediate protection against persistent murine norovirus infection Advances in Inflammatory Bowel Diseases 2013 Fellows Research Meeting : 2013.
Vesselin Tomov, Lisa Osborne, David Artis, and E. John Wherry: Epitope-Specific T Cell Responses to Enteric Murine Norovirus Infection Center for Molecular Studies in Digestive and Liver Diseases at the University of Pennsylvania 2012 Symposium : 2012.
Vesselin Tomov, Lisa Osborne, Douglas Dolfi, Gregory Sonnenberg, Laurel Monticelli, David Artis, and E. John Wherry: Epitope-Specific T cell Responses to Enteric Murine Norovirus (MNV) Infection University of Pennsylvania 2012 Immunology Graduate Group Retreat : 2012.
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