Description of Research Expertise:
Major focus of my research are
1. Development of genital herpes vaccine
2. Development of new animal models, or modulation of existing animal models to learn and evaluate treatment and prevention options for genital herpes, and HIV co-infections.
3. Point of Care diagnostic for HSV-2 infections for resource limited settings.
Expertise in vaccine development.
General virology techniques including, production, purification and titration of virus.
Strategy to create mutant viruses and in-vivo evaluation.
Expertise in development of animal models for evaluation of infectious agents and vaccines.
Developed humanized mouse model for HSV-2 genital infections.
Evaluation of immune responses.
Evaluation and blocking of evasion of host immune response.
Hook LM, Cairns TM, Awasthi S, Brooks BD, Ditto NT, Eisenberg RJ, Cohen GH, Friedman HM: Vaccine-induced antibodies to herpes simplex virus glycoprotein D epitopes involved in virus entry and cell-to-cell spread correlate with protection against genital disease in guinea pigs PLoS Pathogen 14 : e1007095,2018 .
Wang S, Mott KR, Cilluffo M, Kilpatrick CL, Murakami S, Ljubimov AV, Kousoulas KG, Awasthi S, Luscher B, Ghiasi H: The Absence of DHHC3 Affects Primary and Latent Herpes Simplex Virus 1 Infection J Virol 92 (4): e01599-17,2018.
Awasthi S., Hook L. M., Shaw C.E. and Friedman H. M.: A trivalent subunit antigen glycoprotein vaccine as immunotherapy for genital herpes in the guinea pig genital infection model. Human Vaccine Immunotherapy 8 : 1-9,2017.
Awasthi S, Friedman HM: Molecular association of herpes simplex virus type 1 glycoprotein E with membrane protein Us9 Archives of Virology 161 (11): 3203-13,2016.
Awasthi S, Mahairas GG, Shaw CE, Huang ML, Koelle DM, Posavad C, Corey L, and Friedman HM: A dual modality HSV-2 vaccine for preventing genital herpes using glycoproteins C and D subunit antigens to induce potent antibody responses and adenovirus vectors containing capsid and tegument proteins as T cell immunogens J Virol 89 (16): 8497-509,2015.
Shih-Chuan Liaoa, Jing Penga, Michael G. Mauka, Sita Awasthi, Jinzhao Songa, Harvey Friedman, Haim Bau, Changchun Liua: Smart Cup: Minimally-Instrumented, Smartphone-Based Point-of-Care Molecular Diagnostics Sensors and Actuators B: Chemical : 2015.
Awasthi S, Huang J, Shaw CE, Friedman HM: Blocking herpes simplex virus 2 glycoprotein E immune evasion as an approach to enhance efficacy of a trivalent subunit antigen vaccine for genital herpes
J Virol 88 (15): 8421-8432,2014.
Awasthi S, Belshe R, Friedman HM: Better neutralization of HSV-1 than HSV-2 by antibody from subjects immunized with the GlaxoSmithKline gD2 vaccine J Infect Dis 210 (4): 571-575,2014.
Awasthi S, Balliet JW, Flynn JA, Lubinski JM, Shaw CE, Distefano DJ, Cai M, Brown M, Smith JF, Kowalski R, Swoyer R, Galli J, Copeland V, Rios S, Davidson RC, Salnikova M, Kingsley S, Bryan J, Casimiro DR, Friedman HM: Protection Provided by an HSV-2 Glycoprotein C and D Subunit Antigen Vaccine against Genital HSV-2 Infection in HSV-1 Seropositive Guinea Pigs J Virol 88 (4): 2000-2010,2014.
Kamoun M, Filali M, Murray MV, Awasthi S, Wadzinski BE: Protein phosphatase 2A family members (PP2A and PP6) associate with U1 snRNP and the spliceosome during pre-mRNA splicing Biochem Biophys Res Commun 440 (2): 306-311,2013.