Description of Research Expertise:
My research interests stem from my clinical interest in sepsis and lung injury. Our lab investigates how red blood cells and lung endothelium modulate innate immune responses. Our current research aims to understand the mechanisms of the programmed cell death pathway necroptosis in lung endothelium and the role of danger signals released during necroptosis in the development of lung injury. We have also focused on defining the immunomodulatory properties of RBCs and their role in the development of lung injury. Our lab utilizes murine models, human cell culture models, patient samples and dynamic microfluidic models of RBC-induced endothelial injury in order to understand how perturbations of normal RBC and endothelial function contribute to lung inflammation and injury.
H. Luke Anderson, Igor E. Brodsky, Nilam S. Mangalmurti: The evolving erythrocyte: RBCs as modulators of innate immunity Journal of Immunology 201 (5): 1343-1351,2018.
Hotz JM, Qing D, Shashaty Michael GS, Zhang P, Faust H, Sondheimer N, Rivella S, Worthen GS, Mangalmurti, NS.: RBCs homeostatically bind mtDNA through TLR9 to maintain quiescence and prevent lung injury American Journal of Respiratory and Critical Care Medicine 197 (4): 470-480, 2018.
Qing D, Conegliano D, Shashaty MG, Seo J, Reilly JP, Worthen GS, Huh D, Meyer NJ, Mangalmurti NS.: Red Blood Cells Induce Necroptosis of Lung Endothelial Cells and Increase Susceptibility to Lung Inflammation American Journal of Respiratory and Critical Care Medicine 190 (11): 1243-54,2014.
Jeongyun Seo1, David Conegliano1, Megan Farrell, Minseon Cho, Xueting Ding, Thomas Seykora, Danielle Qing, Nilam S. Mangalmurti, Dongeun Huh: A microengineered model of RBC transfusion-induced pulmonary vascular injury Scientific Reports 7 (1): 3413,2017.
Shashaty MGS, Reilly JP, Sims C, Holena DN, Qing D, Meyer NJ, Lanken PN, Feldman HI, Christie JD, Mangalmurti NS: Plasma levels of receptor interacting protein kinase-3 (RIP3), an essential mediator of necroptosis, are associated with acute kidney injury in critically ill trauma patients. Shock 46 (2): 139-143,2016.
Mangalmurti NS, Friedman JL, Wang LC, Stolz DB, Muthukumaran G, Siegel DL, Schmidt AM, Lee JS, Albelda SM: The Receptor for Advanced Glycation End Products Mediates Lung Endothelial Activation by RBCs Am J Physiol Lung Cell Mol Physiol 304 (4): L250-63,2013.
Mangalmurti Nilam S, Chatterjee Shampa, Cheng Guanjun, Andersen Emily, Mohammed Aishat, Siegel Donald L, Schmidt Ann Marie, Albelda Steven M, Lee Janet S: Advanced glycation end products on stored red blood cells increase endothelial reactive oxygen species generation through interaction with receptor for advanced glycation end products. Transfusion 50 (11): 2353-61,2010.
Mangalmurti Nilam S, Xiong Zeyu, Hulver Mei, Ranganathan Mrunalini, Liu Xiang Hong, Oriss Timothy, Fitzpatrick Meghan, Rubin Marc, Triulzi Darrell, Choi Augustine, Lee Janet S: Loss of red cell chemokine scavenging promotes transfusion-related lung inflammation. Blood 113 (5): 1158-66,2009.
Christie JD, Shah CV, Kawut SM, Mangalmurti N, Lederer DJ, Sonett JR, , Ahya VN, Palmer SM, Wille K, Lama V, Shah PD, Shah A, Weinacker A, , Deutschman CS, Kohl BA, Demissie E, Bellamy S, Ware LB: Plasma Levels of Receptor for Advanced Glycation End-Products (sRAGE), Blood Transfusion, and Risk Of Primary Graft Dysfunction American Journal of Respiratory and Critical Care Medicine 180 (10): 1010-1015,2009.
Yani Zhao, Nilam Mangalmurti, Zeyu Xiong, Bharat Prakash, Fengli Guo, Donna B. Stolz, and Janet S. Lee: Duffy antigen receptor chemokines mediates chemokine endocytosis through a macropinocytosis-like process in endothelial cells. Plos One 6 (12): e29624,2011.