Histological Services

All costs are listed below.

• Tissue Processing and Paraffin Embedding
• Paraffin Sectioning
• OCT/Frozen Sectioning
• Staining
  • H&E
  • Alcian Blue
  • Oil Red O
  • PAS
  • Trichrome

Training

Training is available for all equipment.

Reagents

No charge to members for reagents listed below:

• Secondary Antibodies
  • Cy2/Alexa Flour 488
    • Anti-Armenian Hamster
    • Anti-Chicken
    • Anti-Digoxigenin
    • Anti-Goat
    • Anti-Guinea Pig
    • Anti-Mouse
    • Anti-Rabbit
    • Anti-Rat
    • Anti-Sheep
  • Cy3
    • Anti-Armenian Hamster
    • Anti-Chicken
    • Anti-Digoxigenin
    • Anti-Goat
    • Anti-Human
    • Anti-Mouse
    • Anti-Rabbit
    • Anti-Rat
    • Anti-Sheep
    • Anti-Syrian Hamster
  • Cy5
    • Anti-Chicken
    • Anti-Goat
    • Anti-Guinea Pig
    • Anti-Mouse
    • Anti-Rabbit
    • Anti-Rat
    • Anti-Sheep
  • Biotinylated
    • Anti-Chicken
    • Anti-Goat
    • Anti-Guinea Pig
    • Anti-Hamster
    • Anti-Human
    • Anti-Mouse
    • Anti-Mouse
    • Anti-Rabbit
    • Anti-Rat
    • Anti-Sheep
    • Anti-Syrian Hamster
• Kits
  • VectaStain Elite ABC kit (DAB reporter, Vector)
  • VectaStain Elite ABC kit (AP reporter, Vector)
  • Avidin / Biotin Blocking kit (Vector)
  • Peroxidase Substrate kit DAB (Vector)
• Additional Reagents
  • DAPI
  • Propidium Iodide
  • Protein Blocker
  • Sudan Black B
  • YoYo

How to Submit Samples

Please place an order using our online tissue order form here.  Specimen IDs’ should be listed in vertical format with one ID per line.

Please create a Unique ID for each sample, 15 characters or less.  We batch process samples and are not responsible for mix-ups if there are duplicate identifiers between your samples and previously submitted samples, other lab members' samples, etc.  We recommend you label each sample with its unique ID on the front and side of the cassette, using either a pencil or a chemical resistant pen.  

Samples should be pre-fixed in individual cassettes, then transferred to a container filled with 70% EtOH and labeled "PI's last name/Contact name” prior to drop off in cold room 965. It is difficult to return glassware so MPIC users are welcome to borrow a beaker.  If providing slide boxes, please drop them off on the shelf labeled "Slide box drop off" in 931 BRB.

You will receive an email when your order is complete and ready to be picked up.

Please contact an MPIC staff member if you have any questions or concerns about submitting your order.

Molecular Pathology & Imaging Core Services are available at subsidized prices to AFCRI Members and Members of the Center for Molecular Studies in Digestive and Liver Diseases. Center Member Application (Word doc)

Services	Center Members/AFCRI Members	UPENN and CHOP (Non-Center Members)
Rush Order	2x charge	2x charge
Tissue Processing and Embedding	$5.00/block	$7.50/block
Frozen or Paraffin Sectioning (Includes up to 7 Slides with 1 or 2 H&E)	$17.50/block	$26.25/block
Additional Sections	$2.50/slide	$3.75/slide
Additional H&E	$2.50/slide	$3.75/slide
Special Stains: PAS, Trichrome, Alcian Blue, Oil Red O	$9.00/slide	$14.00/slide
RNase-Free Sectioning	Additional $2.50 / slide	Additional $3.75/slide
Spinning Disk Confocal Microscope Use	$20.00/hour ($100.00 maximum/day)	N/A
Laser Capture Microscope Use	$20.00/hour	N/A
Leica BOND RXm Use	$6.00/slide	$9.00/slide
X-Clarity Tissue Clearing System	$20.00/day	$20.00/day
Custom Services/Sectioning (Discuss in advance with MPIC staff)	$140/hour

Microscope

• Fluorescent & Brightfield
  • Two Nikom E600 microscopes with Brightfield, Nomarski and Fluorescent optics. Z-series and deconvolution.
  • Nikon E600 equipped with Brightfield and Nomarski optics.
• Widefield Fluorescent
  • Nikon Eclipse Ti-U microscope with environmental chamber and warming plate for live-cell imaging. Capable of imaging DAPI, FITC, Cy3, Cy5, and brightfield color with an automated stage. Equipped with image stitching capabilities and Metamorph software.
• Laser capture Microdiscetion
  • Nikon Eclipse TE2000-S with Brightfield, Phase Contrast, Fluorescent optics and imaging.
• Inverted Leica DM IRB microscope with Brightfield, Phase Contrast and Fluorescent optics.
• Dissecting Microscope
  • Nikon SMZ-U dissecting microscope with imaging.
  • Nikon SMZ645 dissecting stereo microscope can be signed out.
• Leica BOND RXm
• X-Clarity
• Pressure Cooker
• Snap Freezer
  

Software

• Nikon NIS-Elements

• iVision

• Metamorph

 

 

Paraffin Sections 

• Immunofluorescence
• IHC
• Pepsin Digestion IHC
• Protease Digestion IHC
• Trypson Digestion IHC

Frozen Sections 

• Immunofluorescence
• IHC

Staining

• H&E

Helpful Links

Other Cores      

School of Medicine Cores

Educational Sites

Microscopy U - Extensive information about microscopy provided by Nikon
ImageJ User Guide - Helpful with learning to use ImageJ

Spectrum Viewers

Invitrogen Spectrum Viewer - Useful in determining the excitation and emission peaks of various fluorophores

Free Software

ImageJ - Imaging software provided by NIH.
Fiji - ImageJ with additional plug-ins

These references are available at MPIC:

• Developmental Biology 8^th Edition, by Gilbert
• Mouse Development: Patterning, Morphogenisis, and Organogenesis, by Rossant Tam
• The Anatomical Basis of Mouse Development, by Kaufman Bard
• Under the Microscope, by Curry
• Advanced Laboratory Methods in Histology and Pathology, by Mikel
• Laboratory Methods in Histotechnology, by Prophet, Mills, Arrington, Sobin
• Techniques in Immunochemistry, by Bullock and Petrusz
• Analytical Morphology: Theory, Applications & Protocols, by Gu
• The Rat Brain, by Klippel
• Histopathological Methods and Color Atlas of Special Stains and Tissue Artifacts, by Luna
• Antibodies: A Laboratory Manual, by Harlow
• Color Atlas of Basic Histology 2^nd Edition, by Berman
• Atlas of Histology with Functional Corrections 8^th Edition, by Eroschenko
• Tumors of the Intestines - Second Series, by Fenoglio-Preiser
• Live Cell Imaging: A Laboratory Manual 2^nd Edition, by Goldman
• The Image Processing Handbook 4^th Edition, by Russ
• Fluorescence Microscopy 2^nd Edition, by Herman
• Handbook of Biological Confocal Microscopy 3^rd Edition, by Pawley
• Poly Scientific R&D Corp Reference Guide
• Fundamentals of Light Microscopy and Electronic Imaging, by Douglas B. Murphy
• Bancroft’s Theory and Practice of Histological Techniques 8^th Edition, by Suvarna, Layton, Bancroft