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Transgenic and Chimeric Mouse Facility

Director: Douglas Epstein, PhD

The Transgenic and Chimeric Mouse Facility (TCMF) provides a centralized service to efficiently produce infection-free transgenic, chimeric, and genome-edited strains of mice. Among the services provided by the TCMF are generation of genetically altered mice via direct genome editing (CRISPR/Cas9, and less frequently Zn finger and TALEN technologies), by DNA microinjections into fertilized oocyte to create transgenic lines, or by generation of chimeric mice via embryonic stem cell injection into blastocysts. The Transgenic and Chimeric Mouse Facility also carries out embryo re-derivation, embryo and sperm cryopreservation, in vitro fertilization (IVF), and centralized cryopreservation storage. The TCMF uses state-of-the-art laser conditioning of the zona to facilitate IVF and has intracytoplasmic sperm injection capability on-line to complement IVF services. Newly developed services include the major expansion of cryopreservation services with corresponding expansion of the cryopreservation facility, integration of CRISPR/Cas9 direct genome modifications with a newly established Perelman School of Medicine CRISPR core, and electroporation of DNA and RNA into embryos to increase throughput and decrease wait time for TCMF services. The Facility is led by Dr. Douglas Epstein, Professor of Genetics.

Contact

Director: Douglas Epstein, PhD (epsteind@pennmedicine.upenn.edu)

Technical Director: Jean Richa, PhD (jricha@pennmedicine.upenn.edu)

For more information, you can visit the Facility's website: https://www.med.upenn.edu/genetics/tcmf/