Jose Pascual Lab
- Jose L. Pascual, MD, PhD, FRCS(C), FRCP(C), FACS
Assistant Professor of Surgery
Jose L. Pascual MD, PhD, FRCS(C), FRCP(C) is a surgeon trained at McGill University with specialized fellowship training in trauma and critical care at the University of Pennsylvania in Philadelphia. His clinical and research interests are in shock and head injury. His research work is shared in clinical and basic science to learn how a variety of insults including head injury and shock affect the microcirculation and how different management strategies influence their effects. This basic science work is translated into several clinical projects both retrospective and prospective evaluating the effects of different osmotherapeutic agents in traumatic brain injured patients as well as the immunemodulating effects of different resuscitation fluids in different forms of shock. Dr. Pascual is also the lead physician in a series of pedagogical efforts to train medical trainees as well as advanced practitioners in the ICU using simulation based curricula.
- Rachel Eisenstadt, BA Candidate
Rachel is an undergraduate student at the University of Pennsylvania and a member of Dr. Jose Pascual's lab. Her project focuses on the effects of immunemodulating fluid therapy on the innate immune response during intra-abdominal sepsis. Specifically she utilizes flow cytometry, cremaster intravital microscopy, and histology to quantify host tissue damage, neutrophil surface expression, rolling, and adhesion in a mouse model of intra-abdominal sepsis.
- Wanfeng Gong, MD, MS
Lab manager — Dr. Pascual's lab
Wanfeng Gong MD, MS is a Research Specialist from China, with 7 years of clinical experience as an ENT doctor. She received a Master degree from the University of Louis Pasteur (ULP) in 2004, France. Her work in the lab focuses on applying the different resuscitation fluids to hemorrhagic shock and head injury animal model and in understanding the immunemodulating effects on the brain microcirculation.
- Kenichiro Kumasaka, MD
Visiting Research Scholar
Kenichiro is a visiting scholar from Japan, with 5 years of clinical experience as critical and emergency care doctor. He is interested in osmotherapy in post traumatic brain injury (TBI). His work in the lab focuses on anti-inflammatory properties of two commonly used osmotherapeutic agent (Hypertonic Saline and Mannitol) in TBI.
- Mohammad A. Murcy, MD
Visiting Research Scholar
Mohammad is visiting research scholar from Egypt, with a 4 year experience of general surgery. Currently, he is doing basic science research studying the different immunomodulatory strategies after Traumatic Brain Injury. Evaluating in vivo through intravital microscopy the real time microcirculation of pia on live rodent brains. Currently studying the role of Progesterone on Traumatic Brain injury.
John A. Wolf Lab
- John A. Wolf
Instructor, Department of Neurosurgery
Dr. John A. Wolf is an Instructor of Neurosurgery at the University of Pennsylvania. Dr. Wolf's research program is highly collaborative, and focuses on a number of broad areas related to Neurotrauma and Repair.
Trained as a computational and systems level neuroscientist, Dr. Wolf's goals are a deeper understanding of the role of diffuse brain injury in disrupting communication between and within brain regions. He believes that functional measures such as in vivo electrophysiology post-injury will most successfully elucidate the disruption that occurs to white matter pathways as well as the circuitry in important cognitive areas such as the hippocampus.
In collaboration with Drs. Smith and Cullen, Dr. Wolf is also leading the electrophysiological efforts to integrate electrodes with nervous tissue in vitro so that it can then be implanted for both peripheral and central nervous system repair (Neural Engineering). He is particularly excited to be adding optogenetic tools for feedback to the neural interface, as well as using them for neuromodulation in vivo. He is also leading the effort to utilize stretch-grown axonal tissue to repair peripheral nerve lesions in a large-mammalian model of peripheral nerve injury.
Dr. Wolf received his Ph.D. in Neuroscience from the University of Pennsylvania, where he focused on computational models of the nucleus accumbens in Dr. Leif Finkel's laboratory. He was then a postdoctoral fellow with Dr. Diego Contreras examining recordings utilizing chronic multiple electrode arrays in awake behaving rats, examining information processing in this area of the brain. His next training was as a postdoctoral fellow at the University of Pennsylvania in the Center for Brain Injury and Repair in the Department of Neurosurgery where he developed a method of slice recording of the porcine hippocampus post traumatic brain injury.
Dr. Wolf is currently an active member of the National Neurotrauma Society and the Society for Neuroscience, the Biomedical Engineering Society, and the International Basal Ganglia Society, as well as a Review Editor for Frontiers in Systems Neuroscience.
John Duda Lab
- John Duda, MD
Director, Parkinson's Disease Research, Education and Clinical Center and Associate Professor of Neurology, Perelman School of Medicine at the University of Pennsylvania
John Duda, MD is the Director of the Parkinson's Disease Research, Education and Clinical Center (PADRECC) of the Philadelphia VA Medical Center and an Associate Professor of Neurology at the Perelman School of Medicine at the University of Pennsylvania. He received his medical doctorate from Thomas Jefferson University and finished his Neurology Residency as Chief Resident of the Hahnemann Medical School. He completed a Neuropathology Post-Doctoral Fellowship at the Center for Neurodegenerative Disease Research at the University of Pennsylvania, where he focused on the role of alpha-synuclein in the pathophysiology of Parkinson's disease and related disorders. Dr. Duda leads a multidisciplinary research effort aimed at understanding the cause and treatment of non-motor symptoms in Parkinson's disease. He has authored more than 60 scientific publications including articles in the journals Science, JAMA, The New England Journal of Medicine, Neuron and Annals of Neurology and is recognized as an expert in the neuropathology of Parkinson's disease and dementia with Lewy bodies.
His current basic science research interests include understanding the earliest pathological alterations that occur in Parkinson's disease, whether traumatic brain injury plays a role in the development of those alterations and developing animal models of the neurodegenerative consequences of traumatic brain injury.