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Culture - duodenal tissue


Definition:

A duodenal tissue culture is a laboratory exam to check a piece of tissue from the first part of the small intestine (duodenum). The test is to look for organisms that cause infection.

Alternative Names:

Duodenal tissue culture

How the Test is Performed:

A piece of tissue from the first part of the small intestine is taken during an upper endoscopy (esophagogastroduodenoscopy).

The sample is then sent to a lab, and placed in a special dish (culture media) that allows bacteria or viruses to grow. The sample is looked at under a microscope regularly to see if any organisms are growing.

Organisms that grow on the culture are identified.

How to Prepare for The test:

This is a test done in a lab. The sample is collected during an upper endoscopy and biopsy procedure (esophagogastroduodenoscopy). Ask your health care provider how to prepare for this procedure.

Why the Test is Performed:

A culture of duodenal tissue is done to check for bacteria or viruses that may lead to certain illnesses and conditions.

Normal Results:

No harmful bacteria or viruses are found.

What Abnormal Results Mean:

An abnormal finding means that harmful bacteria or a virus has been found in the tissue sample. Bacteria may include:

  • Campylobacter
  • Helicobacter pylori
  • Salmonella
Considerations:

Other tests are very often done to look for infection-causing organisms in duodenal tissue. These tests include the urease test (for example, the Clotest) and histology (looking at the tissue under a microscope).

Routine culture for H. pylori is not currently recommended.

References:

DuPont HL. Approach to the patient with suspected enteric infection. In: Goldman L, Schafer AI, eds. Cecil Medicine. 24th ed. Philadelphia, PA: Saunders Elsevier; 2011:chap 291.

Semrad CE. Approach to the patient with diarrhea and malabsorption. In: Goldman L, Schafer AI, eds. Cecil Medicine. 24th ed. Philadelphia, PA: Saunders Elsevier; 2011:chap 142.

Giannella RA. Infectious enteritis and proctocolitis and bacterial food poisoning. In: Feldman M, Friedman LS, Brandt LJ, eds. Sleisenger & Fordtran's Gastrointestinal and Liver Disease. 9th ed. Philadelphia, PA: Saunders Elsevier; 2010:chap 107.

Croft AC, Woods GL. Specimen collection and handling for diagnosis of infectious diseases. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 22nd ed. Philadelphia, PA: Saunders Elsevier; 2011:chap 63.

Salwen MJ, Siddiqi HA, Gress FG, Bowne WB. Laboratory diagnosis of gastrointestinal and pancreatic disorders. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 22nd ed. Philadelphia, PA: Saunders Elsevier; 2011:chap 22.

Fritsche R, Selvarangan R. Medical parasitology. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 22nd ed. Philadelphia, PA: Saunders Elsevier; 2011:chap 62.


Review Date: 5/15/2014
Reviewed By: Jenifer K. Lehrer, MD, Department of Gastroenterology, Frankford-Torresdale Hospital, Aria Health System, Philadelphia, PA. Review provided by VeriMed Healthcare Network. Also reviewed by David Zieve, MD, MHA, Isla Ogilvie, PhD, and the A.D.A.M. Editorial team.

The information provided herein should not be used during any medical emergency or for the diagnosis or treatment of any medical condition. A licensed physician should be consulted for diagnosis and treatment of any and all medical conditions. Call 911 for all medical emergencies. Links to other sites are provided for information only -- they do not constitute endorsements of those other sites. Copyright 2002 A.D.A.M., Inc. Any duplication or distribution of the information contained herein is strictly prohibited.

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